Phenotypes of dental remains are remarkable characteristics that can provide a variety of data on human populations. This study focuses on non-metric dental traits analysis and statistical analysis of the Neolithic population at the Nong Ratchawat archaeological site (4000 - 3000 BP), the Ban Kao culture in West-Central Thailand excavated by the 2nd Regional Office of Fine Arts Department, Suphanburi. Trait analyses include shovelling, double shovelling, spine, interruption grooves, dental tubercles, De Terra’s tubercles, Carabelli’s cusps, protostylids, and the groove pattern Y and X of permanent teeth. The project aims to group population affinities at Nong Ratchawat, including comparative analysis with the other prehistoric samples in Southeast Asia.